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. 2018 Dec;13(12):2200–2208. doi: 10.4103/1673-5374.241472

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Copyright: © Neural Regeneration Research

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Effect of Huangqin flavonoid extraction on the expression of neurofilament-H (NF-H) and brain-derived neurotrophic factor (BDNF) in neurons in the injured spinal cord of SCI rats.

(A) NF-H (TRITC, red) and BDNF (FITC, green) expression with double-immunofluorescence labeling, and 4′,6-diamidino-2-phenylindole (DAPI) counterstaining, of the injured spinal cord at 3, 14 and 28 d of recovery in each group. (B) The integrated optical density of NF-H and BDNF double-immunofluorescence expression at 3, 14 and 28 d of recovery in each group (n = 8 per group). Western blot results (C) and bar graphs (D) showing NF-H and BDNF expression at 3, 14 and 28 d of recovery in each group (n = 6 per group). Data are presented as the mean ± SD, and analyzed by one-way analysis of variance followed by the Student-Newman-Keuls post hoc test. **P < 0.01, vs. normal group; #P < 0.05, ##P < 0.01, vs. model group. N: Normal group; M: model group; H: Huangqin group; BDNF: brain-derived neurotrophic factor; NF-H: neurofilament-H; DAPI: 4′,6-diamidino-2-phenylindole; d: days.