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. 2018 Dec;13(12):2200–2208. doi: 10.4103/1673-5374.241472

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Copyright: © Neural Regeneration Research

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Effect of Huangqin flavonoid extraction on the expression of glial fibrillary acidic protein (GFAP) and CD11b in astrocytes and microglia in the injured spinal cord of SCI rats.

(A) GFAP (DyLight 594, red) and CD11b (Dylight 488, green) expression with double-immunofluorescence labeling, and DAPI counterstaining, of the injured spinal cord at 3, 14 and 28 d of recovery in each group. (B) The integrated optical density of GFAP and CD11b double-immunofluorescence expression at 3, 14 and 28 d recovery in each group (n = 8 per group). Western blot results (C) and bar graphs (D) showing GFAP (50 kDa) and CD11b (127 kDa) expression at 3, 14 and 28 d of recovery in each group (n = 6 per group). Data are presented as the mean ± SD, and analyzed by one-way analysis of variance followed by the Student-Newman-Keuls post hoc test. **P < 0.01, vs. normal group; #P < 0.05, ##P < 0.01, vs. model group. N: Normal group; M: model group; H: Huangqin group; GFAP: glial fibrillary acidic protein; DAPI: 4′,6-diamidino-2-phenylindole; d: days.