FIGURE 5.

Predominant abundance of CD45^LoCD11b⁺ cells in hCX3CR1^I249/M280 mice at peak phase of disease. (A) Gating strategy of brain mononuclear cells separated over Percoll gradients at peak and chronic stage of disease. Cells were stained and analyzed by flow cytometry, singlets were gated and cells separated to distinguish microglia (CD45^LoCD11b⁺), neutrophils (CD45^HiCD11b⁺Ly6G⁺), macrophages (CD45^HiCD11b⁺Ly6C^HiLy6G^-), monocyte derived DCs (CD45^HiCD11b⁺Ly6G^-CD11c⁺) and conventional DCs (CD45^HiCD11b^-Ly6G^-CD11c⁺). (B–E) Frequency of total infiltrating cells, microglia, and myeloid subsets at peak and chronic stage of disease are shown, each point represents data from an individual mouse. ^∗∗p < 0.01.