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. 2018 Nov 1;309:132–142. doi: 10.1016/j.jneumeth.2018.09.004

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 1 — Optimization of the liquid-based assay. (A) Worms were exposed to the indicated concentrations of PTZ in solution for 15 min and then the proportion of worms experiencing head-bobbing convulsions (unc-25, unc-49) or full body convulsions (unc-43) in 30 s was scored. unc-49 mutants exhibited the highest incidence of seizure-like activity and were selected for further optimization. (B and C) unc-49 mutants were exposed to the indicated concentrations of PTZ for 15 min and then both the proportion of worms exhibiting head-bobbing convulsions (B) and the number of convulsions experienced per animal (C) was measured over 30 s. (D and E) unc-49 mutants were exposed to 7 mg/ml PTZ for the indicated times and then both the proportion of worms exhibiting head-bobbing convulsions (D) and the number of convulsions experienced per animal (E) was measured over 30 s. Data shown were pooled from three independent experiments (n = 27–30 worms in total per concentration or time point).