Fig. 2.

Responses to PTZ are not age-dependent in younger worms.

unc-49 mutants were age-synchronised by bleaching and grown to the indicated ages before being exposed to 7 mg/ml PTZ in solution for 15 min. At this point, the proportion of worms exhibiting head-bobbing convulsions (A) and the number of convulsions experienced per animal (B) were measured over 30 s. No age-associated difference in PTZ responsiveness was seen when the proportions of animals exhibiting convulsions were scored (A); but the mean number of convulsions per worm (B) was significantly reduced in 4-day-old adults as compared to younger ages of days 1 and 2. Data shown were pooled from three independent experiments (n = 27–30 worms in total per adult age point). Statistical analysis was performed with the Kruskal-Wallis test with Dunn's correction for multiple comparisons; *, p < 0.05.