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. 2018 Oct 17;9:2476. doi: 10.3389/fmicb.2018.02476

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Copyright © 2018 Xu, Li, Yu, Liu, Zhang, Liu, Pan and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of SsSte12 silencing strains. (A) Generation of SsSte12 silencing constructs. Two fragement he SsSte12 in gene coding regions were used to generate the knockdown mutants pSD2 and pSD3 by gene silencing strategy. (B) SsSte12 expression levels in wild-type strain UF1, empty vector transformant pSD-EV and silenced mutants (pSD2-1, pSD2-4, pSD2-11, and pSD2-12; pSD3-1, pSD3-4, pSD3-7, and pSD3-8). qRT-PCR was performed to profile the SsSte12 expression. Gene expression values are normalized relative to the constitutively expressed histone gene. Values are the means ± SD (n = 3; ^∗∗P < 0.01; one-way ANOVA).