Table 1.
Bacterial Strains and Plasmids Used in This Study
| Strain | Description | References |
|---|---|---|
| Staphylococcus aureus | ||
| Newman | MSSA; WT strain isolated from a human infection in 1952 | 17 |
| Newman ΔmprF | Newman mprF::Em; Emr | 26 |
| CS295L | Newman ΔmprF strain expressing mprFS295L | 9 |
| CS295L+L826F | Newman ΔmprF strain expressing mprFS295L+L826F | This study |
| CT345A | Newman ΔmprF strain expressing mprFT345A | 9 |
| CT345A+L826F | Newman ΔmprF strain expressing mprFT345A+L826F | This study |
| CL826F | Newman ΔmprF strain expressing mprFL826F | This study |
| Escherichia coli DH5α | Host strain for construction of recombinant plasmids | 36 |
| Plasmid | ||
| pRB474 | Shuttle vector carrying Bacillus subtiltis vegII promoter; Cmr | 18 |
| pCR2.1 | E. coli plasmid; Ampr | Invitrogen |
Ampr, ampicillin resistance; CL, cardiolipin; Cmr, chloramphenicol resistance; Emr, erythromycin resistance.