Table 2.

In Vitro Susceptibility Assays, Lysyl-Phosphatidylglycerol Synthesis, and Daptomycin Binding Assays of the Study Strains

			% survival (mean ± SD) after 2 h exposure to:
Strain	mprF SNP	DAP MICs (μg/ml)	hNP-1 (10 μg/ml)	LL-37 (1 μg/ml)	Amount (μg) of bound DAP (5 μg)
Newman	—	0.5	34.8 ± 7.7	36.4 ± 4.7	0.72 ± 0.12
Newman ΔmprF	—	0.125	5.0 ± 2.9a	2.1 ± 1.6a	1.32 ± 0.11a
	S295 L	1	28.2 ± 5.4	32.8 ± 6.4	0.62 ± 0.12
	S295 L+L826F	0.38	19.7 ± 6.2b	10.8 ± 4.6c	0.98 ± 0.20b
	T345A	2	28.5 ± 7.7	26.7 ± 3.6	0.59 ± 0.18
	T345A+L826F	0.38	17.8 ± 6.5b	6.8 ± 2.4c	0.84 ± 0.23b
CL826F	L826F	0.75	—	—	—

^ap < 0.01 versus Newman wild-type (WT) strain.

^bp < 0.05 versus C_S295L and C_T345A strains, respectively.

^cp < 0.01 versus C_S295L and C_T345A strains, respectively.

DAP, daptomycin; MICs, minimum inhibitory concentrations; SD, standard deviation; SNPs, single nucleotide polymorphisms.