Fig. 1.

Construction and confirmation of the mutant strains deficient in two hfq genes. (A) Agarose gel electrophoresis from the PCR assays for confirmation of the transformation of the two hfq genes. The transformed pVIK112 vector with the insertion of an hfq1-like gene was missing in the wild-type Burkholderia glumae BGR1, and confirmed in the HFQ1 mutant strain, and the 445 bp within the hfq2-like gene was absent in the HFQ2 deletion strain, in comparison with wild-type B. glumae BGR1. M, 100 bp DNA ladder (B) Agarose gel electrophoresis from RT-PCR was performed for confirmation of the two hfq gene-expression in wild-type B. glumae BGR1 and deficient mutant strains HFQ1, HFQ2, and HFQ12. M, 100 bp DNA ladder; C, B. glumae BGR1 genomic DNA.