Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristics | References |
|---|---|---|
| B. glumae | ||
| BGR1 | Wild-type, Rif | (Jeong et al., 2003) |
| BGS2 | BGR1 tofI::Ω | (Kim et al., 2004) |
| HFQ1 | BGR1 hfq1::Ω | This study |
| HFQ2 | BGR1 Δhfq2 | This study |
| HFQ12 | BGR1 hfq1::Ω Δhfq2 | This study |
| HFQ1C | HFQ1 harboring pRK_H1 | This study |
| HFQ2C | HFQ2 harboring pRK_H2 | This study |
| HFQ12C | HFQ12 harboring pRK_H12 | This study |
| E. coli | ||
| DH5α λpir | For cloning and replication of plasmids | Lab collection |
| S17-1 λpir | For transferring cloning plasmids to recipient cells | Lab collection |
| Plasmids | ||
| pK18mobsacB | Allelic exchange suicide vector, sacB, Km | |
| pVIK112 | R6K suicide vector, lacZY for transcriptional fusions, Km | |
| pRK415 | Broad host range expression vector, Tet | |
| pVIK_Hfq1 | pVIK112 vector cloning internal region of hfq1 | This study |
| pK18_Hfq2 | pK18mobsacB vector cloning LR fragment of hfq2 | This study |
| pRK_H1 | pRK415 vector including hfq1 gene | This study |
| pRK_H2 | pRK415 vector including hfq2 gene | This study |
| pRK_H12 | pRK415 vector including hfq1 and hfq2 gene | This study |