Amplifying internal region of hfq1 |
Hfq1_F |
AAAAGAATTCGGAGTACGCCATGAGCAACA
|
Amplifying internal region of hfq1 |
Hfq1_R |
AAAAGGTACCGGAGCAACACGACGTACTGG
|
Confirming vectors and mutants |
Lacfuse_R |
GGGGATGTGCTGCAAGGCG |
Confirming for mutants |
Hfq1_Up_F |
TAGTGTAGCGATTGACGGCG |
Amplifying L fragment of hfq2 |
Hfq2 _LF |
AAAAGAATTCTTTTCTCGGGTCACATCCGC
|
Amplifying L fragment of hfq2 |
Hfq2 _LR |
AAAGGATCCCATTGAGAAGCGGCTTGAGC
|
Amplifying R fragment of hfq2 |
Hfq2 _RF |
AAAGGATCCAATCGCACGAAGGCGTCTC
|
Amplifying R fragment of hfq2 |
Hfq2 _RR |
AAAAAGCTTGAAACGCGGATGTGAAGACG
|
Confirming for mutants |
Hfq2_Gene_Up_F |
GACCCGAAGAACCCCGACTA |
Confirming for mutants |
Hfq2_Gene_Down_R |
CCAGAAGGCACAGACGGACC |
Confirming for mutants |
pk18_Down_R |
GTGAAGCTAGCTTATCGCCAT |
RT-PCR |
RT_Hfq1_F3 |
CGATTGACGGCGGATCTCTT |
RT-PCR |
RT_ Hfq1_R2 |
TTAGGAGGACGCGCCTTCCG |
RT-PCR |
RT_ Hfq2_F2 |
ATGGCCAATCCTGCAGAATCC |
RT-PCR |
RT_ Hfq2_R2 |
TTACTCGTTGCTGCCGCCGG |
Amplifying hfq1 to add in pRK415 |
C_ P2-F |
AAAAAGCTTCGGCAATCAGGATTCGCATG
|
Amplifying hfq1 to add in pRK415 |
C_ P2-R |
AAGGTACCGAATCGTCCTTTGCTATGATGA
|
Amplifying hfq1 to add in pRK415 |
C_kpn1_Hfq1_F |
AAGGTACCATGGAGTACGCCATGAGCAA
|
Amplifying hfq1 to add in pRK415 |
C_BamH1_Hfq1_R |
AAAGGATCCGATAAGGCGGCTGGCGTGGA
|
Amplifying hfq2 to add in pRK415 |
C_EcoR1_Hfq2_F |
AAAAGAATTCGTTTTCTCGGGTCACATCCG
|
Amplifying hfq2 to add in pRK415 |
C_BamH1_Hfq2_R |
AAAGGATCCAGATGCAGGTCCAGTCGCT
|