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. 2018 Oct 1;34(5):412–425. doi: 10.5423/PPJ.OA.06.2018.0097

Table 2.

Primers used in this study

Purpose Primer name Sequence (5’ to 3’)
Amplifying internal region of hfq1 Hfq1_F AAAAGAATTCGGAGTACGCCATGAGCAACA
Amplifying internal region of hfq1 Hfq1_R AAAAGGTACCGGAGCAACACGACGTACTGG
Confirming vectors and mutants Lacfuse_R GGGGATGTGCTGCAAGGCG
Confirming for mutants Hfq1_Up_F TAGTGTAGCGATTGACGGCG
Amplifying L fragment of hfq2 Hfq2 _LF AAAAGAATTCTTTTCTCGGGTCACATCCGC
Amplifying L fragment of hfq2 Hfq2 _LR AAAGGATCCCATTGAGAAGCGGCTTGAGC
Amplifying R fragment of hfq2 Hfq2 _RF AAAGGATCCAATCGCACGAAGGCGTCTC
Amplifying R fragment of hfq2 Hfq2 _RR AAAAAGCTTGAAACGCGGATGTGAAGACG
Confirming for mutants Hfq2_Gene_Up_F GACCCGAAGAACCCCGACTA
Confirming for mutants Hfq2_Gene_Down_R CCAGAAGGCACAGACGGACC
Confirming for mutants pk18_Down_R GTGAAGCTAGCTTATCGCCAT
RT-PCR RT_Hfq1_F3 CGATTGACGGCGGATCTCTT
RT-PCR RT_ Hfq1_R2 TTAGGAGGACGCGCCTTCCG
RT-PCR RT_ Hfq2_F2 ATGGCCAATCCTGCAGAATCC
RT-PCR RT_ Hfq2_R2 TTACTCGTTGCTGCCGCCGG
Amplifying hfq1 to add in pRK415 C_ P2-F AAAAAGCTTCGGCAATCAGGATTCGCATG
Amplifying hfq1 to add in pRK415 C_ P2-R AAGGTACCGAATCGTCCTTTGCTATGATGA
Amplifying hfq1 to add in pRK415 C_kpn1_Hfq1_F AAGGTACCATGGAGTACGCCATGAGCAA
Amplifying hfq1 to add in pRK415 C_BamH1_Hfq1_R AAAGGATCCGATAAGGCGGCTGGCGTGGA
Amplifying hfq2 to add in pRK415 C_EcoR1_Hfq2_F AAAAGAATTCGTTTTCTCGGGTCACATCCG
Amplifying hfq2 to add in pRK415 C_BamH1_Hfq2_R AAAGGATCCAGATGCAGGTCCAGTCGCT