Fig. 2.

Ndufs4(KO) inhibits short-term potentiation (STP) after high frequency stimulation and leads to short-term depression in the presence of isoflurane. (a) The depiction of the experimental flow. Axons from CA3 are stimulated and postsynaptic potentials in CA1 are recorded. (b) Representative traces recorded during baseline, 2 min after high-frequency stimulation (HFS) and 20 min after HFS in wild-type and Ndufs4(KO) hippocampal slices in the absence of isoflurane. (c) In the absence of isoflurane, compared with wild-type, there is less STP after HFS in Ndufs4(KO) slices [control: n=10 slices, Ndufs4(KO): n=9 slices]. (d) There are small, but statistically significant, differences in field excitatory postsynaptic potentials (fEPSPs) during HFS trains in the absence of isoflurane. We interpret those differences as not biologically substantial. (e) Representative traces recorded during baseline, 2 min after HFS and 20 min after HFS in wild-type and Ndufs4(KO) slices in 0.25 mM isoflurane. (f) Ndufs4(KO) slices pre-exposed to 0.25 mM isoflurane show pronounced short-term depression after HFS [control: n=8 slices, Ndufs4(KO): n=9 slices]. (G) There are no differences between genotypes in fEPSPs during the first HFS train. fEPSPs in Ndufs4(KO) slices do not recover after the first and second HFS train as evidenced by the smaller than baseline initial fEPSPs of the second and third trains. Here and in subsequent figures, the black downward arrow represents the first HFS train, and error bars represent standard error of the mean, unless stated differently. Red dots show statistically significant differences between wild-type and Ndufs4(KO) slices.