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. 2018 Oct 24;13(10):e0202297. doi: 10.1371/journal.pone.0202297

Fig 2. Effect of irbesartan pre-treatment on oxidative stress, on cardiac marker BNP and on TLRs mRNA expression.

Fig 2

(A) The release of peroxynitrite was measured by Griess assay. (B) mRNA levels BNP was evaluated by RT-qPCR. (C) Western blot analysis of BNP protein, blot is representative from three independent experiments. (D) TLR2 and (E) TLR4 mRNA expressions were measured by RT-qPCR. TLR2 an TLR4 mRNA levels were normalized relative to GAPDH mRNA levels. Data represent the mean ± S.E.M. of three independent experiments (*P<0.05 versus CTRL; #P<0.05 versus hypoxia, § P<0.05 versus irbesartan 10 μM).