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. 2018 Sep 28;10(10):2813–2822. doi: 10.1093/gbe/evy215

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — —GiTim17 is localized in proximity to GiTim44. (A) BAP-tagged GiTim17 (green) is biotinylated in vivo by the HA-tagged cytosolic BirA (red). (B) The proteins chemically cross-linked to GiTim17 by DTME were copurified and analyzed by mass spectrometry. (Top) The detection of biotinylated GiTim17 in the fractions derived from the protein purification. HSP—the initial high-speed pellet fraction, W1 and W2—wash steps, E—eluate from the streptavidin-coated Dynabeads. (Bottom) The SDS-PAGE gel of the elute. (C) Identified proteins were ordered according to the enrichment score. Only proteins enriched more than three times are shown (the complete list of proteins is shown in supplementary table 1, Supplementary Material online). Putative new mitosomal proteins are shown in red letters.