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. Author manuscript; available in PMC: 2018 Oct 24.
Published in final edited form as: Cell Physiol Biochem. 2017 Nov 23;44(2):701–715. doi: 10.1159/000485284

Fig. 4.

Fig. 4.

Effect of Y-27632 on actin/SRF/myocardin pathway in TA VSMCs. (A) Effects of Y-27632 on ratio of F/G actin detected by western blotting. (B) Fluoresce staining of F- and G-actin in primary WKY rat thoracic aorta (WTA) and SHR thoracic aorta (STA) VSMCs. Scale bar: 50μm. (C) The mRNA level of myocardin in WTA and STA VSMCs. (D) Representative western blots and the quantified data of myocardin in nuclear fractions and total cell lysates from WTA and STA VSMCs and the ratio of nuclear/total protein. (E) The mRNA level of SRF in WTA and STA VSMCs. (F) Representative Western blots and the quantified data of SRF in nuclear fractions from WTA and STA VSMCs. GAPDH and HDAC1 were used as loading control of total cell lysates and nuclear fractions, respectively. Data are shown as mean ± SEM, **P<0.01 vs WTA, ##P<0.01 vs corresponding vehicle. NS: no significant difference.