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. Author manuscript; available in PMC: 2018 Oct 24.
Published in final edited form as: Cell Physiol Biochem. 2017 Nov 23;44(2):701–715. doi: 10.1159/000485284

Fig. 5.

Fig. 5.

Effects of Y-27632 on the expression of stiff-ness-associated genes in VSMCs. (A) The relative mRNA levels of SRF/myocardin target genes in VSMCs, α-SMA: al pha-smooth muscle ac-tin; MYH11: myosin heavy chain 11; SM22: smooth muscle 22; SMTN: smoothelin. (B) Represen-tative western blots and the quantified data of α-SMA in total cell lysates. GAPDH was used as loading control. (C) Immunostainings of α-SMA in VSMCs. Scale bar: 50μm. Data are shown as mean ± SEM, **P<0.01 vs WTA, ##P<0.01 vs corresponding vehicle. All, n=5 rats/group.