Fig. 5. Brain concentrations of Δ⁹-trans-THC and PET diastereoisomers and induced biochemical changes.

LC-MS/MS quantification of (A) Δ⁹-trans-THC (red), cis-PET (green), and trans-PET (blue); (B) AEA; (C) 2-AG; (D) AA; (E) PGD₂; and (F) PGE₂ in the brain of male BALB/c mice 1 hour after intraperitoneal injection. The effects of PET diastereoisomers on PGD₂ and PGE₂ were inhibited by the CB1 receptor antagonist rimonabant (SR1). Doses are expressed in mg/kg. Data show means ± SD. Groups were compared with the vehicle-treated control group using a one-way ANOVA following Tukey’s post hoc test n = 5 to 15 mice per group. **P < 0.01, *P < 0.05 versus vehicle or as reported by the arches. ns, not significant.