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. 2018 Oct 24;4(10):eaau5716. doi: 10.1126/sciadv.aau5716

Fig. 5. DMSP promotes Sulfitobacter D7 virulence toward E. huxleyi.

Fig. 5

Time course of algal and bacterial growth in cultures of (A and B) the sensitive E. huxleyi strain 379 and (C and D) the resistant E. huxleyi strain 2090, monocultures (dotted lines) and during coculturing with Sulfitobacter D7 (solid lines). Cultures were supplemented at day 0 with 100 μM of the following substrates: DMSP (green, triangle), glycerol (yellow, square), propionate (purple, diamond), or none (gray, circle). Inset in (B): zoomed-in view of bacterial growth at days 2 to 5. No bacterial growth was observed in control cultures. Results represent average ± SD (n = 3). Error bars smaller than the symbol size are not shown. Statistical differences were tested using two-way repeated-measures ANOVA, accounting for infection and the different substrates. (A) P < 0.001 for the differences between control and cocultures and for the differences between the DMSP treatment and the other treatments in cocultures. (B) P < 0.05 only for the differences between the glycerol treatment and the rest of the treatments in cocultures. (C) P < 0.001 for the differences between the DMSP treatment in cocultures and the other treatments. (D) P < 0.01 for the differences between all treatments in cocultures.