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. 2018 Sep 29;11(9):1349–1356. doi: 10.14202/vetworld.2018.1349-1356

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Copyright: © Prihatno, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-3 — The structure of seminiferous tubules after secretome injection in the low- and high-dose groups (hematoxylin and eosin staining; 500×). 1 week after secretome injection, few spermatogonia (red arrow) and spermatozoa (black arrow) were observed in the low-dose group (A1), and few primary spermatocytes (blue arrow) were observed in the high-dose group (A2). The regenerative effects were observed in both groups 1 week after the second secretome injection. That is, the spermatogenic cells were complete. However, spermatozoa were denser in the seminiferous tubules (B2) of the high-dose group than in those of the low-dose group (B1). 1 week after the third secretome injection, low- and high-dose group (C1 and C2) showed complete spermatogenic cells. However, no spermatozoa were noted in the low-dose group. 1 week after the fourth secretome injection in the high-dose group (D1), the complete stage of spermatogenic cells and spermatozoa was observed in the lumen of the seminiferous tubules.