Fig. 4. Increase in HA-specific antibody levels following immunization with HAd-H5HA with a decline in vector immunity with time.
At 1, 3, 6, and 10 months post-HAd-GFP inoculation, naïve or HAdV-primed BALB/c mice were immunized i.m. with 108 PFU of HAd-H5HA, and at 4 weeks after the immunization with HAd-H5HA, the blood samples were collected to monitoring the development of HA-specific antibody levels by ELISA. ns, non-significant at p<0.05; *, significant at p<0.05; and ****, significant at p<0.0001. The statistical analysis was done by Bonferroni post-test and two-way ANOVA using GraphPad Prism 6.