Figure 1.

Click-A+Chip is a graphene-based biosensor for the digital identification of proteins after bio-orthogonal non-canonical amino acid tagging of the young proteome in the setting of heterochronic parabiosis. Click-A+Chip is composed of a field effect biosensor with a graphene channel which is functionalized with a novel synthetic molecule capable of selectively binding to ANL-labeled proteins via click-chemistry. The binding events between the linker molecule and the ANL-labeled protein result in the alteration of graphene conductivity and biosensor electrical parameters which can be detected using a handheld analyzer. Click-A+Chip is also capable of protein identification by further utilizing a range of primary antibodies to identify the labeled proteins within a few minutes and without the need for any secondary antibodies or any complex instrumentation.