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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Neurosci Methods. 2018 Aug 3;308:183–191. doi: 10.1016/j.jneumeth.2018.08.002

Figure 2: Schematics of Microfludic Device Fabrication Process.

Figure 2:

The master mold for the device consist of two layers of photoresist structures. The first layer defines the microchannels at 5 μm height. The second layer defines the culture chambers at 100 μm height. Soft lithography is used to make PDMS device, which is then assembled with glass coverslip as bottom and coated with PLO-laminin. The three chambers are connected by two sets of microchannels. The middle chamber is accessed through the two circular reservoirs.