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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Neurosci Methods. 2018 Aug 3;308:183–191. doi: 10.1016/j.jneumeth.2018.08.002

Figure 4: Microfluidic device shows controlled protein transport and robust axon crossing.

Figure 4:

A) Varying the loading volume in each chamber changes the amount of protein transported. 50/50/110 correspond to the volumes in somal/middle/distal chamber in μL. With a volume increase in the distal and middle chamber, the amount of fluorescence increased more than 2-fold (6.6% to 13.8%). *: p<0.05 N=4. B) Quantification of axons crossing the middle chamber with different conditions. Both conditions showed robust axons crossing (-/G100 at 64.6%±10.7%, -/G700 at 55.3%±15.2%). N = 8.