Figure 1.

Binding of FH6,7/Fc (10 µg/ml) to six strains of N. gonorrhoeae and their lgtA mutants. FH6,7/Fc binding to wild-type (wt) N. gonorrhoeae strains F62, Ctx-r(Spain), H041, MS11, UMNJ60_06UM (abbreviated to ‘NJ-60’) and FA1090 by flow cytometry is shown by the grey shaded histograms and binding to their respective lgtA mutants is shown by the histograms depicted by solid lines. Conjugate controls for wt strains and lgtA mutants are shown by dotted and dashed lines, respectively. Numbers alongside histograms in grey shaded boxes and clear boxes represents the median fluorescence of binding to wt strains and lgtA mutants, respectively. In every instance, the median fluorescence of the conjugate controls was less than 10. X-axis, fluorescence on a log₁₀ scale; Y-axis, counts. One representative experiment of two reproducible independently performed experiments is shown.