Figure 8.
Efficacy of FH6,7/Fc against N. gonorrhoeae strain OC7 in human FH/C4BP transgenic (Tg) mice. Premarin®-treated FH/C4BP dual Tg mice (n=10/group) were given 6.2 × 107 CFU strain OC7 intravaginally on Day 0 and treated intravaginally daily with 10 µg/d of either FH6,7/Fc (dashed grey lines and triangles) or FH6,7/Fc with D270A/K322A mutations that abrogates C1q binding (solid grey lines and open inverted triangles) or 10 µl/d PBS (control; solid black line and circles) from Day 0 to Day 10. Vaginas were swabbed daily to enumerate CFU. A Kaplan Meier curves showing time to clearance of infection. Groups were compared using the log-rank (Mantel-Cox) test. Significance was set at 0.017 (Bonferroni’s correction for comparisons across three groups). B. Log10 CFU versus time. X-axis, day; Y-axis, log10 CFU. C. Bacterial burdens consolidated over time (Area Under the Curve [log 10 CFU] analysis). The three groups were compared using the non-parametric Kruskal-Wallis equality of populations rank test. The χ2 with ties (two degrees of freedom) was 19.8 (P=0.0001). Pairwise comparisons across groups was made with the Two-sample Wilcoxon rank-sum (Mann-Whitney) test.