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. 2018 Oct 17;38(5):BSR20181059. doi: 10.1042/BSR20181059

Figure 2. Selection of genes for downstream validation.

Figure 2

(A) A549 cells were transduced with lentiviral virus to express MERS-N and LacZ. Cells were subsequently selected in blasticidin, and protein expression was analyzed at day 2 and 10 post-selection by IFA using mAb 7H6. (B) RNA transcripts were obtained from A549-transduced cells expressing MERS-N or LacZ at day 2 and 10 post-selection and assayed using an 84-gene antiviral PCR array. Gene regulation was analyzed with a cutoff of 4-fold change and genes up-regulated on both day 2 and 10 post-selection are represented in a Venn diagram. The intersection shows common genes up-regulated on both days.