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. 2018 Oct 17;38(5):BSR20181059. doi: 10.1042/BSR20181059

Figure 5. MERS-N regulates CXCL10 through its C-terminal fragment.

Figure 5

(A) 293FT cells were transiently transfected with empty vector, FLAG-tagged N, N1-195, and N196-413. Western blot analysis was performed using anti-FLAG and anti-GAPDH antibodies. (B) RNA transcripts were obtained from transiently transfected 293FT cells and assayed by RT-qPCR to analyze the mRNA fold changes of CXCL10. (C) Cell supernatants were harvested from transiently transfected 293FT cells, and CXCL10 secretion was evaluated using ELISA. The results of these experiments were expressed as mean ± S.D. (error bars) of three independent experiments in triplicates. Asterisk (*) indicates statistical significance of P<0.05 when compared with vector-transfected cells at the respective time-points.