Figure 7. The influence of depletion of SKA2 on biological effects in breast cancer cells.
(A) The influence of depletion of SKA2 on the ability of cell proliferation in breast cancer cells. (B) Representative images indicated that the invading cells with down-regulated SKA2 analyzed by Transwell assays; scale bar: 50 μm. (C) Quantitative results are illustrated for (B). (D) The influence of depletion of SKA2 on cell migration and invasion was measured by wound healing assays. (E) The influence of depletion of SKA2 on the cell cycle in breast cancer cells. The influence of depletion of SKA2 on cell cycle was analyzed by propidium iodide staining and flow cytometry. Bar plots illustrating the percentage of G0/G1 and S phase in breast cancer cells with depletion of SKA2. (F) Quantitative results are illustrated for (E). (G) The influence of depletion of SKA2 on apoptosis in breast cancer cells. The influence of depletion of SKA2 on cell apoptosis was evaluated by AnnexinV/PI staining and flow cytometry. (H) Bar plots illustrating the percentage of apoptosis cells in breast cancer cells with depletion of SKA2. (I) Effects of depletion of SKA2 on cell cycle and apoptosis related factors in breast cancer cells. The influence of depletion of SKA2 on the mRNA level of cell cycle and apoptosis-related factors were analyzed by qRT-PCR. (J) Western blot analyzed the protein level of cell cycle and apoptosis-related factors in breast cancer cells with depletion of SKA2. The data are the results of three independent experiments and are presented as mean ± S.D. *P and ##P<0.05; **P<0.05 or ***P<0.01. ‘si-SKA2’ is short for ‘si-SKA2-1’.