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. 2018 Oct 23;38(5):BSR20181325. doi: 10.1042/BSR20181325

Figure 8. Proteolytic susceptibility of P. gingivalis HmuY and T. forsythia Tfo.

Figure 8

(A) Both proteins in apo- and holo-forms in their native states (native) and after thermal denaturation (denatured) were subjected to trypsin digestion and visualized by staining with CBB G-250. Susceptibility of proteins to P. gingivalis (B) or T. forsythia (C) proteases was examined by growing bacterial cells under high- (Hm) or low-iron/heme (DIP) conditions in the presence of the purified HmuY or Tfo proteins (marked with asterisks). Protein samples collected at indicated time points were separated by SDS/PAGE and visualized by staining with CBB G-250 (B). (D) The presence of Tfo in P. gingivalis cultures was also examined by Western blotting using rabbit polyclonal anti-Tfo antibodies.