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. 2018 Oct 24;9:4428. doi: 10.1038/s41467-018-06528-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Gene clusters and correlation of locus-1 to NAS-C biosynthesis. a Biosynthetic gene clusters with CDPS and P450 in CMB-MQ030. Locus-1 and 2 are resembling only except locus-1 contains an additional TP (transporter) gene; locus-3 containing MT (methyltransferase), CDO-A (cyclodipeptide oxidase A), and CDO-B (cyclodipeptide oxidase B) is homologous to alb biosynthetic gene cluster. b HPLC analysis the production of NAS-C in CMB-MQ030 and locus-1 expression S. albus. (I) Standard NAS-A, (II) standard NAS-B, (III) CMB-MQ030, (IV) mWHU2475 (S. albus containing locus-1), and (V) standard NAS-C spiked with standard NAS-B from the co-injection of standard NAS-C and -B