Table 1.
Abundance ratio and putative function of differentially expressed proteins identified by MALDI-TOF MS.
| Spot ID | Protein Name | Locus ID | MS | Theoretical | Experimental | MP/PS | SC% | Abundance ratio | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pI | Mr | pI | Mr | Nootripathu/IR20 | High Bulk/Low Bulk | ||||||
| D1 | Putative ADP-ribosylation factor | LOC_Os03g27450.1 | 60 | 5.42 | 22.0 | 4.47 | 42 | 6/23 | 38 | 0.43 ± 0.07 | 0.43 ± 0.01 |
| D2 | Cell wall adhesion | LOC_Os11g11730.1 | 78 | 4.45 | 48.5 | 4.84 | 35 | 11/21 | 38 | 0.49 ± 0.09 | 0.53 ± 0.13 |
| D3 | Retrotransposon protein | LOC_Os01g54700.1 | 58 | 7.8 | 20.5 | 4.55 | 12 | 12/30 | 43 | 0.33 ± 0.03 | 0.49 ± 0.02 |
| D6 | Auxin response factor | LOC_Os06g47150.4 | 73 | 8.67 | 52.3 | 5.81 | 39 | 8/23 | 17 | 1.00 ± 0.13 | 0.84 ± 0.41 |
| D7 | Expressed protein | LOC_Os01g56790.1 | 63 | 5.95 | 16.4 | 5.82 | 36 | 7/24 | 52 | 0.37 ± 0.02 | 0.37 ± 0.23 |
| D8 | Oxidoreductase, aldo/ketoreductase family protein | LOC_Os02g03100.2 | 61 | 9.16 | 28.5 | 5.19 | 12 | 7/36 | 34 | 0.33 ± 0.12 | 0.24 ± 0.03 |
| D9 | Maturase K | LOC_Os04g16734.1 | 58 | 9.8 | 33.8 | 5.52 | 17 | 22/35 | 49 | 0.53 ± 0.10 | 0.64 ± 0.12 |
| D10 | Chloroplast 30S ribosomal protein S3 | LOC_Os04g16824.1 | 76 | 10.0 | 25.3 | 6.45 | 42 | 10/13 | 43 | 0.47 ± 0.11 | 0.34 ± 0.07 |
| D11 | MAR-binding filament-like protein 1 | LOC_Os01g08510.1 | 72 | 5.83 | 80.2 | 6.40 | 53 | 21/36 | 25 | 0.54 ± 0.07 | 0.47 ± 0.08 |
| D12 | Proteophosphoglycan ppg4 | LOC_Os05g10830.2 | 71 | 12.2 | 25.1 | 6.89 | 47 | 7/36 | 36 | 0.52 ± 0.02 | 0.53 ± 0.05 |
| D13 | Disease resistance protein RPM1 | LOC_Os11g12040.1 | 67 | 6.3 | 77.9 | 5.90 | 52 | 30/33 | 39 | 0.53 ± 0.03 | 0.39 ± 0.05 |
| D14 | PPR repeat domain containing protein | LOC_Os10g36190.1 | 62 | 7.47 | 55.1 | 6.78 | 38 | 15/25 | 19 | 0.44 ± 0.09 | 0.63 ± 0.22 |
| D15 | Ras-related protein | LOC_Os02g37420.1 | 61 | 6.9 | 23.4 | 4.40 | 43 | 12/23 | 71 | 0.37 ± 0.05 | 0.47 ± 0.02 |
| U1 | Hexokinase | LOC_Os07g09890.1 | 47 | 6.49 | 55.0 | 4.40 | 47 | 5/13 | 11 | 1.88 ± 0.05 | 2.13 ± 0.25 |
| U2 | Protein kinase family protein | LOC_Os08g02050.1 | 68 | 8.37 | 58.6 | 4.41 | 59 | 14/14 | 13 | 2.40 ± 0.33 | 2.43 ± 0.25 |
| U3 | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase | LOC_Os10g28360.1 | 60 | 5.07 | 22.1 | 4.63 | 60 | 6/30 | 33 | 4.53 ± 0.02 | 2.15 ± 0.04 |
| U4 | Peroxiredoxin | LOC_Os07g44430.1 | 48 | 5.97 | 22.2 | 4.50 | 85 | 4/20 | 31 | 17.19 ± 0.2 | 3.38 ± 0.18 |
| U6 | Annexin | LOC_Os07g46550.1 | 68 | 6.2 | 20.1 | 5.60 | 90 | 9/18 | 36 | 1.77 ± 0.21 | 2.01 ± 0.08 |
| U7 | Leucine rich repeat containing protein | LOC_Os08g40090.1 | 41 | 6.83 | 62.3 | 6.52 | 57 | 21/22 | 24 | 2.56 ± 0.02 | 2.08 ± 0.10 |
| U9 | Expressed protein | LOC_Os04g24610.1 | 70 | 4.88 | 56.3 | 4.60 | 24 | 8/29 | 25 | 2.05 ± 0.20 | 1.81 ± 0.55 |
| U10 | Pathogenesis-related Bet v I family protein | LOC_Os03g18850.1 | 63 | 6.04 | 12.9 | 6.05 | 53 | 5/20 | 34 | 2.06 ± 0.09 | 2.03 ± 0.01 |
| U11 | CGMC_MAPKCMGC_2_ERK.13 - CGMC includes CDA, MAPK, GSK3, and CLKC kinases | LOC_Os08g06060.1 | 55 | 8.05 | 42.9 | 6.00 | 54 | 8/20 | 32 | 1.91 ± 0.15 | 2.71 ± 0.33 |
| U12 | Glutaredoxin | LOC_Os05g28530.1 | 60 | 9.28 | 26.5 | 6.40 | 56 | 6/18 | 25 | 2.03 ± 0.01 | 1.66 ± 0.13 |
| U13 | RNA recognition motif | LOC_Os04g50790.1 | 53 | 5.85 | 33.5 | 6.36 | 38 | 10/28 | 31 | 1.73 ± 0.02 | 1.93 ± 0.64 |
| U14 | Expressed protein | LOC_Os12g06560.1 | 76 | 6.39 | 117.9 | 5.22 | 48 | 17/22 | 15 | 2.54 ± 0.11 | 1.79 ± 0.06 |
| U15 | Leaf senescence related protein | LOC_Os01g68630.1 | 50 | 5.69 | 67.3 | 5.49 | 54 | 7/25 | 14 | 2.13 ± 0.47 | 1.54 ± 0.06 |
| U16 | Ras-related protein | LOC_Os07g31370.1 | 75 | 8.58 | 21.1 | 6.95 | 64 | 8.42 | 44 | 9.73 ± 1.19 | 2.36 ± 0.05 |
| U17 | NADPH reductase | LOC_Os09g38620.1 | 65 | 5.38 | 79 | 6.22 | 50 | 11/40 | 17 | 1.59 ± 0.05 | 2.35 ± 0.89 |
| U18 | NBS-LRR disease resistance protein | LOC_Os11g45180.1 | 71 | 6.26 | 118 | 6.84 | 59 | 11/32 | 13 | 1.59 ± 0.35 | 1.99 ± 0.46 |
Homologous proteins were identified by MASCOT analysis along with their experimental and theoretical pI and molecular weight (Mr). Corresponding accession numbers were obtained by performing BLAST search in the TIGR database (http://rice.plantbiology.msu.edu/analyses_search_blast.shtml). High bulk represents the bulk of RILs possessing high root penetration ability, and low bulk represents the bulk of RILs possessing low root penetration ability. Where MS is Mascot score, MP/PS is peptide matched/peptide searched, SC% is sequence coverage percentage; abundance ratios in bold fonts indicate significant at p < 0.05.