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. 2018 Oct 24;8:15685. doi: 10.1038/s41598-018-34115-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AGO2 associates with nascent RNA genome-wide. (A) Binary Heatmap comparing genes positive for AGO2 ChIP-seq or ChRIP-seq. Black bar corresponds to genes bearing AGO2 in both ChIP-seq and ChRIP-seq assays. (B) Screenshot showing the example gene cv-2, which is up-regulated by neuRNA-seq in AGO2 knockdown and whose TSS is located in active RED chromatin. Black bar corresponds to the AGO2 called ChIP-seq peak.