Fig. 3.

A. Protein alignment of CntA and YeaW showing sequence conservation of the functional domains based on representative CntA sequences from functional confirmed Citrobacter freundii CntA, Acinetobater baumanii CntA, Escherichia coli CntA and Escherichia coli YeaW. The alignment shows a high level on sequence conservation between CntA sequences and the functional domain of YeaW is indistinguishable. B. Phylogenetic tree of Rieske oxygenases based on full length protein sequences with bootstrap values. A. and B. combined show that the CntA from Escherichia coli SE11 [17] and YeaW from Escherichia coli DH10B [26], [27] have an identical protein sequence. CntA of Citrobacter freundii and Acinetobater baumanii cluster with the other CntA proteins and are separate from other Rieske oxygenases used as outgroups.