REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-VEGFR1	Sigma Aldrich	Cat# V4762 RRID:AB_477622
Mouse monoclonal anti-VEGFR2	Sigma Aldrich	Cat# V9134 RRID:AB_477630
Goat polyclonal anti-Neuropilin-1	Santa Cruz	Cat# SC7239 RRID:AB_2150835
Rabbit monoclonal anti-VEGFR2 phosphoY1212	Cell Signalling Technology	Cat# 2477S RRID:AB_331374
Chemicals, Peptides, and Recombinant Proteins
VEGF165a	R&D Systems (Abingdon, UK)	Cat# 293-VE
VEGF165b	R&D Systems (Abingdon, UK)	Cat# 3045-VE
VEGF121a	R&D Systems (Abingdon, UK)	Cat# 4644-VS
VEGF145a	R&D Systems (Abingdon, UK)	Cat# 7626-VE
VEGF189a	R&D Systems (Abingdon, UK)	Cat# 8147-VE
VEGF111a	R&D Systems (Abingdon, UK)	Cat# 5336-VE
VEGF-Ax	R&D Systems (Abingdon, UK)	Cat# 9018-VE
HaloTag AlexaFluor 488 membrane impermeant substrate	Promega Corporation (Wisconsin, USA)	Cat# G1002
bisBenzimide H 33342 trihydrochloride	Sigma Aldrich	Cat# B2261
Formaldehyde solution 4%	Sigma Aldrich	Cat# F8775
Cediranib	Sequoia Research Products	Cat# SRP01883c
Chromasolv	Sigma Aldrich	Cat# 34877
Rhodamine 6G	Sigma Aldrich	Cat# R4127
Triton-X-100 (laboratory grade)	Sigma Aldrich	Cat# X100
DTT 1,4-Dithiothreitol	Sigma Aldrich	Cat# DTT-RO
PNGase F	Promega Corporation (Wisconsin, USA)	Cat# V4831
Protease-free bovine serum albumin	Milpore	Cat# 126609
Protease-free bovine serum albumin	Sigma Aldrich	Cat# 03117332001
Secondary chick anti-mouse	Invitrogen	Cat# A21463
Secondary donkey anti-goat	Invitrogen	Cat# A11056
Secondary chick anti-rabbit AlexaFluor-488	ThermoFisher Scientific, USA	Cat# A-21441
Chicken serum	Sigma Aldrich	Cat# C5405
Donkey serum	Sigma Aldrich	Cat# D9663
ProLong Gold antifade reagent	ThermoFisher Scientific, USA	Cat# P10144
Dulbecco’s Modified Eagle’s Medium	Sigma Aldrich	Cat# D6429
Fetal Bovine Serum	Sigma Aldrich	Cat# F2442
Medium 200 (Gibco)	ThermoFisher Scientific, USA	Cat# M-200-500
Large Vessel Endothelial Supplement (LVES 50x) (Gibco)	ThermoFisher Scientific, USA	Cat# A1460801
Poly-D-Lysine hydrobromide	Sigma Aldrich	Cat# P6407
Dulbecco’s Phosphate Buffered Saline (DPBS)	Sigma Aldrich	Cat# D8537
Trypsin-EDTA solution x10	Sigma Aldrich	Cat# T4174
Critical Commercial Assays
HaloTag Mammalian Protein Detection and Purification System	Promega Corporation (Wisconsin, USA)	Cat# G6795
ONE-Glo™ Luciferase	Promega Corporation (Wisconsin, USA)	Cat# E6120
Nano-Glo luciferase assay system (Furimazine)	Promega Corporation (Wisconsin, USA)	Cat# N1130
Experimental Models: Cell Lines
Human: GloResponse™ NFAT-RE-luc2P HEK293 cell line (female)	Promega Corporation (Wisconsin, USA)	Cat# E8510
Human: HUVEC cells (newborn male, single donor)	ThermoFisher Scientific	Cat# C0035C. Lot number: 1606186.
Human: HEK293T cells (female)	ATCC (Virginia, USA)	Cat# CRL-3216
Recombinant DNA
NanoLuc-VEGFR2	Promega Corporation (Wisconsin, USA)	Custom synthesis
NanoLuc-NRP1	Promega Corporation (Wisconsin, USA)	Custom synthesis
NanoLuc-NRP1 Y297A	Promega Corporation (Wisconsin, USA)	Custom synthesis
HaloTag-VEGFR2	Promega Corporation (Wisconsin, USA)	Custom synthesis
HaloTag-NRP1	Promega Corporation (Wisconsin, USA)	Custom synthesis
HaloTag-NRP1 Y297A	Promega Corporation (Wisconsin, USA)	Custom synthesis
VEGF165a	Gene Dynamics LLC (Oregon, USA)	Custom synthesis
VEGF165b	Gene Dynamics LLC (Oregon, USA)	Custom synthesis
VEGF121a	Gene Dynamics LLC (Oregon, USA)	Custom synthesis
pFN21 HaloTag CMV Flexi Vector (modified to contain a IL-6 secretion sequence and a EPTTEDLYFQCDN linker sequence)	Promega Corporation (Wisconsin, USA)	Cat# G2821
Software and Algorithms
GraphPad Prism 7.02	GraphPad Software, La Jolla California USA	www.graphpad.com
Zen 2010	Zeiss, Germany	www.zeiss.com
MetaXpress	Molecular Devices, USA	www.moleculardevices.com
Other
Black 96-well plates	Greiner Bio-One	Cat# 655090
White 96-well plates	Greiner Bio-One	Cat# 655098
8-well plates	Nunc Lab-Tek, Thermo Fisher Scientific	Cat# 155411
Coverslips (18x18mm; 1.5H)	Zeiss, Germany	Cat# 474030-9000-000