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. 2018 Oct 24;8:15686. doi: 10.1038/s41598-018-33908-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of proliferation and cell morphological change in Hela229 cells treated with matrine derivatives 1 and 2 for 48 h. Photographs marked with capital letters were obtained from IPCM (inverted phase contrast microscopy), with small letters from FM (fluorescence microscopy); magnification was 200X . (A) Morphological characteristics of cell in control; (a) Morphological characteristics of cell nucleus in control; (B) Morphological changes of cell induced by matrine; (b) Nucleus morphological changes of cell induced by matrine; (C) Morphological changes of cell induced by matrine derivative 1; (c) Nucleus morphological changes of cell induced by matrine derivative 1; (D) Morphological changes of cell induced by matrine derivative 2; (d) Nucleus morphological changes of cell induced by matrine derivative 2.