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. 2018 Oct 24;8:15720. doi: 10.1038/s41598-018-34105-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D visualisation of the two largest sheaths found in all regions investigated. (a) The largest sheath with a demonstrable connection to an artery highlighted in white colour in R1 (view from last section of the series). (b) Same sheath as in (a) at higher magnification and after removal of non-connected structures. (c) The largest sheath without demonstrable connection to an artery highlighted in white colour in R10 (view from last section of the series). (d) Same sheath as in (c) at higher magnification and after removal of non-connected structures. (a–d) show staining for CD34 plus SMA in yellow, for post-arteriolar CD271⁺ capillary sheaths in green and for capillary sheaths of undetectable location in blue. In (a) and (c) CD271⁺ FDCs in a follicle are also blue. The direct connections of arterial vessels to green sheaths were manually marked in red. Length of the horizontal part of the bounding box = 1 mm in (a) and (c); 596 µm in (b) and 293 µm in (d). f = follicle, t = trabecula, tv = trabecular vein, v = vein.