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. 2018 Oct 24;8:15711. doi: 10.1038/s41598-018-34148-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of Pgrmc1 increases hepatic TG accumulation. Normal diet and high fat diet were fed for 1 month to WT and Pgrmc1 KO mice. (A) Lipid droplets are characterized in H&E staining (Scale bar, 50 µm). (B) Oil Red O staining presents TG accumulation in hepatocytes (Scale bar, 25 µm). (C) Quantification of Oil Red O was analyzed by Image J as setting red cells for positive standard. (D) Total lipid from liver was extracted by folch method and level of TG was measured by colorimetric method in 550 nm. (E) Level of TG (triglycerides) in plasma showed inverse proportional level compared to liver TG. (F) Level of FFA in plasma was increased in Pgrmc1 KO mice. Values represent means ± SD. *P < 0.05. Number of mice used in experiment is at least 3 for each group.