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. 2018 Oct 24;8:15711. doi: 10.1038/s41598-018-34148-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Liver-specific regulation of Pgrmc1 controls hepatic fat accumulation through SREBP-1. (A,B) Palmitic acid (330 µM) and oleic acid (660 µM) were incubated for 48 hrs with high glucose medium. Fatty acid substantially increased Oil Red O stained area and AG-205 ameliorated fat accumulation in hepatocyte (Scale bar, 10 µm). (C,D) Fatty acids were incubated in same concentration as above for 4 hrs after pre-incubation of AG-205 for 12 hrs. PGRMC1 mRNA level was increased after AG-205 treatment and SREBP-1 mRNA level was inversely changed to PGRMC1 mRNA level. (E,F) Cells were isolated from mice and maintained in high glucose medium for one day. Fatty acids (Palmitic acid; 330 µM, Oleic acid 660 µM) were treated for 24 hrs. Levels of mature SREBP-1 protein were increased in loss of Pgrmc1. Rplp0 mRNA was used as an internal control in real time PCR. Beta actin was used as an internal control in western blot. Values represent means ± SD of at least 3 experiments. *P < 0.05.