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. 2018 Oct 24;8:15705. doi: 10.1038/s41598-018-33411-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative effect of hydrodynamic focusing and acoustic focusing using unlysed diluted blood from a healthy subject. Representative population distributions for diluted whole blood showing red blood cells (RBCs) and platelets (PTLs) obtained with hydrodynamic instrument (A) (displayed in dotplot A) with hydrodynamic instrument (B) (displayed in dotplot B) with acoustic-assisted focusing turned off (displayed in dotplot C) and with acoustic focusing turned on (displayed in dotplot D). Forward scatter vs. side scatter dotplots display well-defined characteristic arch-shaped populations of erythrocytes only when acoustic focusing is turned on.