FIGURE 6.

AMPK mediated the NAD⁺-induced increases in the intracellular ATP levels of BV2 microglia under basal conditions. (A) Dorsomorphin, an AMPK inhibitor, prevented NAD⁺- induced ATP increases in BV2 cells. The cells were co-treated with 15 μM dorsomorphin and NAD⁺ for 3 h before the ATP assay were conducted. (B) AMPK siRNA treatment led to a significant decrease in the AMPK protein level of BV2 cells. (C) AMPK siRNA significantly attenuated the NAD⁺-induced ATP increases of BV2 cells. The cells were pretreated with AMPK siRNA for 24 h. Subsequently, the cells were treated with NAD⁺ for 3 h. N = 12. The data were pooled from four independent experiments. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001.