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. 2018 Oct 18;12:343. doi: 10.3389/fncel.2018.00343

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Copyright © 2018 Zhang, Wang, Shi, Wu and Ying.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AMP treatment significantly increased the intracellular levels of ATP, ADP, and AMP of BV2 microglia under basal conditions. (A) AMP treatment significantly increased the intracellular ATP level of the cells. (B) AMP treatment significantly increased the intracellular ADP level of the cells. (C) AMP treatment significantly increased the intracellular AMP level of the cells. (D) AMP treatment dose-dependently increased the AMPK phosphorylation of the cells. (E) AMP treatment significantly increased ACC phosphorylation. (F) AMP treatment did not influence the intracellular AMP/ATP ratios. The cells were treated with AMP for 3 h. Subsequently, the assays were conducted. N = 16. The data were pooled from four independent experiments. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001.