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. 2018 Oct 18;12:239. doi: 10.3389/fnbeh.2018.00239

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Copyright © 2018 Liu, Chen, Liu, Wang, Huang, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The timeline and bar graph showing reduced c-Fos expression in the gently handled group compared with the non-handled group in response to LS. (B) Representative images showing c-Fos expression in the SC of gently handled or non-handled groups in response to LS (SL, superficial layer of SC; IL, intermediate layer of SC; DL, deep layer of SC); Scale bars = 100 μm. Mann–Whitney U test, ^∗∗P < 0.01, U = 2; ^∗P < 0.05, U = 4.