Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 5;293(42):16324–16336. doi: 10.1074/jbc.RA118.004226

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Turek et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 4. — Pairing of selected E2s with different types of E3s. A–F, SLCA of PUB20 composed of U-box (yellow) and ARM repeats (A), PUB24 composed of U-box (yellow) and ARM repeats (B), PUB4 composed of UND domain, U-box (yellow) and ARM repeats (C), PUB13 composed of UND domain, U-box (yellow) and ARM repeats (D), MIEL1 composed of two zinc fingers and a RING domain (red) (E), and AvrPtoB composed of two kinase-interacting domains and a U-box-like domain (purple) (F). E3s were assayed against UBC1 (group III), UBC5 (group IV), UBC9 (group VI), UBC12 (group VI), UBC17 (group VII), UBC26 (group XI), UBC28 (group VI), UBC30 (group VI), UBC35 (group XV), and COP10 transiently co-transformed in Arabidopsis mesophyll protoplasts. Transformation efficiencies were normalized by Renilla Luc harbored in the E2-containing vector. Strongest signal intensities are shown in blue. Values indicate the average of three independent biological experiments ± S.D. Statistically significant differences indicated by different letters were determined by one-way ANOVA and Tukey post hoc test (p < 0.05).