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. 2018 Sep 5;293(42):16324–16336. doi: 10.1074/jbc.RA118.004226

Figure 5.

Figure 5.

Activation of the immune response by flg22 treatment modulates E2–PUB22 pairing. A, SLCA of cLuc-PUB22 with nLuc-UBC26 transiently co-transformed in Arabidopsis mesophyll protoplasts and treated with the indicated concentrations of flg22 for 1 h. B, SLCA of nLuc-PUB22 with cLuc-UBC35 transiently co-transformed in Arabidopsis mesophyll protoplasts and treated with the indicated concentrations of flg22 for 1 h. C, SLCA of nLuc-PUB22 with cLuc-UBC30 transiently co-transformed in Arabidopsis mesophyll protoplasts and treated with the indicated concentrations of flg22 for 1 h. A–C, transformation efficiencies were normalized by Renilla Luc harbored in the E2-containing vector. Values indicate the average of three independent biological experiments ± S.D. Statistically significant differences indicated by different letters were determined by one-way ANOVA and Tukey post hoc test (p < 0.05).