Figure 2.

ZnT8–antibody complexes Size-exclusion HPLC chromatograms of ZnT8 in complex with mAb20, Fab20, and/or mAb39 in various combinations and binding stoichiometries are shown. Eight distinct peaks were resolved, labeled 1–8. Corresponding complex molecular weights and predicted complex compositions are summarized in Table 1. mAb20/Fab20 and mAb39 were fluorescently labeled and detected either in the green (shown as blue traces) or red (red traces) channel of a multichannel fluorescence detector. Gray traces, unbound ZnT8 detected by UV absorption. a.u., arbitrary units.