Table 1.
Binding parameters of HTLV-1 proteins to HTLV-1 gRNA-derived constructs
| RNA |
Kd (μm) |
|
|---|---|---|
| MA | NC | |
| ssDNA20a | 1.2 ± 0.3 | 0.6 ± 0.1 |
| SL(450–470)a | 8.1 ± 2.6 | NB |
| SL(474–508)a | 1.9 ± 0.2 | NB |
| SL(447–512)a | 1.6 ± 0.2 | — |
| Rb | 2.8 ± 0.3 | Weak |
| U5b | 3.2 ± 0.3 | 2.7 ± 0.4 |
| 5′ gagb | 2.1 ± 1.0 | Weak |
| 5′ leaderb | 2.5 ± 0.3 | 1.9 ± 0.2 |
a Apparent equilibrium dissociation constants (Kd) determined from FA assays performed in 20 mm Hepes, pH 7.5, 40 mm NaCl, 10 mm KCl, and 1 mm MgCl2. Values are the average of at least three measurements with standard deviations indicated. NB indicates that no binding was detected with up to ∼10 μm protein. A dash indicates that the value was not determined.
b Apparent Kd values determined from EMSAs performed in 20 mm Hepes, pH 7.5, 60 mm NaCl, 10 mm KCl, 1 mm MgCl2, and 5% (v/v) glycerol. Values are the average of at least two measurements with standard deviations indicated. “Weak” indicates that binding affinity could not be determined.