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. 2018 Aug 28;293(42):16364–16375. doi: 10.1074/jbc.RA118.004073

Figure 6.

Figure 6.

Blockade of mTORC2 signaling with PP242 attenuates UUO nephropathy in mice. Male CD1 mice were performed with UUO and sacrificed at day 14 after surgery. A, Western blot analysis showing FN and α-SMA protein abundance in the UUO kidneys from mice treated with vehicle and PP242, respectively. Numbers indicate individual animals within each group (top). Graphic presentation showing the protein abundance for FN and α-SMA in the UUO kidneys (bottom). *, p < 0.05 compared with vehicle group (n = 4). B, representative micrographs showing Masson, periodic acid–Schiff (PAS), and Sirius red staining, as well as immunostaining for FN, α-SMA, Ly6b, and F4/80 in the contralateral (CTL) and UUO kidneys from various groups as indicated. Scale bar, 20 μm. C–F, graphic presentation showing the fibrotic area (C), total collagen content (D), neutrophils (E), and macrophages (F) in kidney tissues among groups as indicated. *, p < 0.05 compared with CTL kidneys (n = 3–6); #, p < 0.05 compared with UUO kidneys with vehicle (n = 3–6). Error bars, S.E.