Figure 4.
DNA-binding studies of PhoP RR with Rv3134c-devR-devS promoter region. A, in silico analysis of the upstream region of Rv3134c-devR-devS operon revealed five putative sites for PhoP binding depicted as 1–5. Regulatory features including DevR binding boxes (P and D) and transcription start sites (aerobic and hypoxic) are indicated. Primers used for amplifying the 218-bp DNA probe for use in EMSAs are shown as half-arrows. B, binding of unphosphorylated DevR and PhoP proteins. C, binding of phosphorylated DevR, PhoP (left), and PhoPRa (right) proteins to target DNA. Arrows, free DNA (F) and bound DNA.