Figure 5.

ADAMTS1 is a direct target gene of miR-362b-3p in HCASMCs. The putative miR-365b-3p targeted sequences in the 3′-UTR of ADAMTS1 mRNA is indicated. Dual-luciferase reporter assay was performed to analyze the relative luciferase activity in HCASMCs. Results are presented as fold change. *P<0.05 vs. miR-365b-3p mimic (3′-UTR-MUT) group. 3′UTR, 3′-untranslated region; PDGF-BB, platelet-derived growth factor-BB; NC, negative control; miR, microRNA; ADAMTS1, a disintegrin and metalloproteinase with thrombospondin motifs 1; HCASMCs, human coronary artery smooth muscle cells.