Figure 1.

Combined treatment with TCS and radiation reduced the survival of SUNE-1 cells in a dose-dependent manner. Human SUNE-1 cells were cultured with DMSO, 4 Gy radiation alone, 20 µM TCS alone, 4 Gy radiation + 10 µM TCS or 4 Gy radiation + 20 µM TCS for 24 h. (A) Morphological differences in SUNE-1 cell colonies were observed under a microscope. Magnification, ×200. (B) Colonies of >50 cells were counted to calculate the survival fraction. **P<0.01 vs. radiation alone. TCS, trichosanthin; DMSO, dimethylsulfoxide.